트라즈올 시약 R1021 R1022 역전사 효소 PCR 시약

트라즈올 시약

TRAzol Reagent is a ready-to-use reagent for the isolation of total RNA from cells and tissues. The reagent, a mono-phasic solution of phenol and guanidine isothiocyanate, is an improvement to the single-step RNA isolation method. During sample homogenization or lysis, TRAzol Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components. Addition of chloroform followed by centrifugation, separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, the RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation. Precipitation with ethanol yields DNA from the interphase, and an additional precipitation with isopropyl alcohol yields proteins from the organic phase. Copurification of the DNA may be useful for normalizing RNA yields from sample to sample.This technique performs well with small quantities of tissue (50-100 mg) and cells (5 × 106), and large quantities of tissue (≥1 g) and cells (>107) of human, animal, plant, or bacterial origin.The simplicity of the TRAzol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA isolated by TRAzol Reagent is free of protein and DNA contamination.

특징

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하류로 흐르는 적용에서 고품질 정제된 전 rna의 오르엘리러블 성능

동셍 생명공학 주식회사는 PCR 기술을 그것의 핵심으로 간주하고 그것의 제품이 공통 PCR, 큐피크레, 핵산 검출과 다른 분야에 초점을 맞춥니다. 연속적 혁신, 선도 기술과 고객 만족도의 끊임없는 추구의 품질 정책을 고수할 때, 우리는 고객들에게 비용 효율적 분자 생물학 제품을 제공합니다.